FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20420832
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112050.038.7157594
CAAGTCG90550.020.81391594
TAAGTCG27550.019.78947494
GAAGTCG96150.019.25949194
CTCGATT36850.015.4328361
GTTTTTT318250.014.8125681
TATACGC6950.014.2014397
CGAAAAC31100.014.0546631
CGCAAAT30100.013.7408641
CGAATCG13350.013.0262173
CTCAGAT155550.012.7206691
CGAAAAT46350.012.6752971
CTCGGAT62700.012.443381
CTTAGAT54450.012.4297511
CGGAAAT40600.012.2709361
CGCAAAC36850.012.2442331
GTTTTAT157700.012.2194031
ATATACG10400.012.2019236
AAATCCA204500.012.1349634
AATCCAA112750.012.1303785
Produced by FastQC (version 0.11.5)