FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21817246
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG117400.056.8483894
TAAGTCG32000.027.46562694
CAAGTCG96950.027.39040894
GAAGTCG133450.022.25852494
AAAGTCG44350.018.65163494
GTTTTTT273300.015.2195391
TTTTTTC341450.014.1640072
CGAAAAT33550.013.3084951
GAAAAAT192200.012.3491141
GTTTAAT81250.012.3212311
GTCGATT21100.012.2511841
GGAAAAT154450.012.1417931
CGCAATT19550.012.020461
TTTTTTG374100.011.9227482
AATCGGG44950.011.815355
GTTTTAC60550.011.64327051
AAATCGA31500.011.6380954
AAAAATC187950.011.6030862
CGGAAAT42100.011.1638961
GTTTTTC157800.010.9904941
Produced by FastQC (version 0.11.5)