FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21817246
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139350.048.16361694
TAAGTCG31750.027.38582494
CAAGTCG109550.026.55682294
GAAGTCG131450.020.95245294
CGCGAAT9700.015.0206191
CGCGTAT4001.469216E-714.11
GCGAAAT27900.013.8136211
GTTTTTT262600.013.4234581
AAAGTCG53050.013.20075494
CGGAAAT40450.012.7812121
CGCGATT6702.910383E-1112.6268661
AAATCGA29450.012.1290324
AAAAATC174850.011.612242
CTCGGAT57800.011.4653981
AAATCCA179050.011.2348514
GTTTTAT133300.010.9654911
GGAAAAT156550.010.8981161
CGCGAAC13400.010.8731351
CGCAATT19550.010.5780051
GTTTAAT71750.010.5463411
Produced by FastQC (version 0.11.5)