FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22967017
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99200.054.912394
CAAGTCG86900.029.74683894
TAAGTCG31650.023.01737894
GAAGTCG114800.018.46428794
GCGGTAT21000.012.5333331
CGCGTAT5302.6446287E-812.4150941
GTCGGAT29550.011.6108291
AAAGTCG47250.011.43915494
GTCGGTT43550.011.0080371
GTCGAAT14950.010.6889631
GGCGGAT92700.010.1402381
TTAGCGA20250.09.9802478
GTCGATA15100.09.9602651
GCCGGTT53550.09.8300661
GTCGTTT23700.09.7172991
GGCGATT41150.09.5941691
CGCGGAT27100.09.5387461
TCGGATT26750.09.487852
GTCCGAT16850.09.483681
TAGCGAG26900.09.2602229
Produced by FastQC (version 0.11.5)