FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22967017
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115550.047.2643994
CAAGTCG102350.026.35857494
TAAGTCG29600.022.86486494
GAAGTCG110950.016.9869394
AAAGTCG50900.012.65029494
GCGGTAT21000.012.0857131
GCGGTTT34200.011.1315791
CGGTATA11900.011.0588242
AATCGTA21500.010.9302325
CGCGTAA6655.3500116E-810.6015031
GCCGGTT48200.010.5311211
CGCAAAT24350.010.4229981
CGCGTAT6353.2308526E-710.3622051
GGGAAAT148150.010.2787711
CGGGGTT92200.010.2462031
CGCGAAT8903.9108272E-1010.0337091
CTCGGTT38050.010.0052571
GTTCGAT24600.09.9349591
GGCGGAT91050.09.8594181
CGTATCG16400.09.7439023
Produced by FastQC (version 0.11.5)