FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22641862
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120550.057.46827394
CAAGTCG93750.027.62346894
TAAGTCG35600.025.21629594
GAAGTCG138650.022.8135694
GTTTTTT311450.015.7245141
TTTTTTC419050.015.5788092
AAAGTCG52300.014.01912194
GTCGATT24550.013.0183311
GTTTAAT94400.012.8453411
AAATCCA204750.012.7858374
TTTTTTG452500.012.4329282
CTCGATT40150.012.4084681
CGAAAAC40400.012.2153461
AAAAATC230800.012.1980082
CGGAAAT48550.012.1977341
GTTTTAC70400.012.1505691
GTTTTTC184500.012.1257461
GAAAAAT226400.012.1029161
CGAAAAT39000.011.930771
GTTTTCT231400.011.7601571
Produced by FastQC (version 0.11.5)