FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22623839
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98900.049.99393594
CAAGTCG75600.025.73809694
TAAGTCG28500.021.60350894
GAAGTCG115700.016.85825394
CGAAAAT38150.014.660551
ATATACG9050.014.5414376
TACGCTA7500.013.7866669
TATACGC7300.013.5205487
CGGAAAT48150.012.4942891
GTCGGTT49500.012.4383841
CGCGAAC14750.012.1084741
GTTTAAT94350.012.00530051
CGAAGAT83400.011.9472431
GTTTTTT294700.011.8337291
AAAGTCG45550.011.76289894
GTTTATT108000.011.5759261
GGAAAAT162950.011.4795951
CTTTATT167800.011.1758051
GGAAAAC148700.011.0625421
CGCGTAT4701.3623438E-511.0000011
Produced by FastQC (version 0.11.5)