FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22623839
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126300.038.70150494
CAAGTCG95400.021.72641694
TAAGTCG31300.021.17252394
GAAGTCG108350.016.83064394
CGAATCG14100.013.3333333
CGAAAAT49250.012.9786821
CGCAAAC46700.012.6809431
CGGAAAT48350.012.0537741
CGCGAAT12800.011.751
CGAAAAC33600.011.7499991
CGAAGAT81050.011.6557681
CGCGTTT16100.011.3850931
GGAAAAT163650.011.1720131
GTTTAAT85300.011.019931
GTCGGTT48500.010.9505161
GTTTATT112450.010.9088491
ATATACG9501.8189894E-1210.8842126
GTTTTTT296350.010.8479841
CTTTAAT102650.010.6225031
AAAGTCG50050.010.61138894
Produced by FastQC (version 0.11.5)