FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21718682
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128800.044.0442694
CAAGTCG98950.026.409394
TAAGTCG27900.021.05734894
GAAGTCG123300.019.97404994
CGCGTAA8200.014.3292691
CGCGTAT4404.6883906E-712.8181831
GTTCGAT27400.011.8357681
CTCGGAT60750.011.6823061
AAAGTCG50800.011.65748194
TCGGATT26400.011.393942
ATCGTAT16200.011.0246926
AATCGTA18450.010.953935
GTCGGTT44250.010.9401131
CTCGGTT40100.010.1970081
CTCGTAT13400.010.1716421
GTCGATT22950.010.0348591
CGTATCG17100.09.8947373
TAGCGAG27600.09.8768129
GTCCGAT15250.09.8622961
TTAGCGA23850.09.853258
Produced by FastQC (version 0.11.5)