FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22412919
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101150.050.41522694
CAAGTCG80350.022.98817694
TAAGTCG30050.018.45590694
GAAGTCG113100.017.57824994
GTTTTTT296850.016.3712311
GCGAAAT29500.015.7728811
TTTTTTC396050.014.6559772
AAAGTCG52750.013.98862594
CGAATTG27850.012.9946143
CGCGAAT18850.012.9655171
GTCGAAT13200.012.8181811
AAATCCA194500.012.6380454
AAATCGA32200.012.5527954
GCGGAAT40850.012.4259491
GAAAAAT224550.012.4119351
GGAAAAT173900.012.2162161
GTTTTTC177200.012.1743781
CGAAAAC34550.012.1070911
AATCCAC116450.011.9871195
GTCGATT20850.011.9472431
Produced by FastQC (version 0.11.5)