FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22412919
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124950.041.03801794
CAAGTCG93200.020.62553894
TAAGTCG31850.018.74097494
GAAGTCG117350.016.86152594
CGAAAAC37300.013.7345851
GTTTTTT283850.013.69350051
CGCAAAC42250.013.4603551
CGCAAAT31050.013.3204511
GCGAAAT28300.012.4558311
AATCGTA19700.012.4060925
AAATCGA37050.012.3049934
CGAATTG29300.012.1911263
CGCGAAT18550.012.1617251
GCCGAAT48550.012.004121
AAAGTCG57550.011.84187694
TTTAATC105550.011.7555662
CTCGATT31550.011.4706811
AAAAATC206050.011.4049982
CGAATCG15700.011.3757963
AAATCCA189450.011.2135134
Produced by FastQC (version 0.11.5)