FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21667002
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135900.058.13612794
CAAGTCG103000.027.60679694
TAAGTCG36700.027.021894
GAAGTCG144200.022.75034794
AAAGTCG52900.017.05860194
GTTTTTT280400.015.2699721
CGAAAAC34200.013.4678351
TTTTTTC356750.013.3062372
TATACGC7600.012.9868437
TCGGATC40150.012.8767132
GCGAAAT27550.012.7949191
GTTTTAC66750.012.60374451
GTTTTAT148900.012.5312291
GTTTTTC159450.012.4979631
CGAAAAT33400.012.3832331
TACGCTA9150.012.3278689
AAAAATC204250.012.1037942
GTTTAAT85250.011.9636361
AAATCCA193600.011.7985534
CTTAGAT50700.011.7731751
Produced by FastQC (version 0.11.5)