FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22805827
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114650.052.02180594
CAAGTCG87900.027.00227594
TAAGTCG33800.026.97633494
GAAGTCG132350.018.43067794
TACGCTA8350.017.4491029
TATACGC7300.016.7397257
AAAGTCG47200.014.93644194
ATATACG11500.011.8521746
CGAATCG16450.011.7142853
CGCGAAT4559.5054E-611.36263751
CTTTAAT104600.011.3231351
GGAAAAT179050.011.0773531
GTCGGTT46750.011.0588241
CGCAATT21800.010.9954131
GTTTTTT291250.010.9895281
GTTTTAC72900.010.7668031
CGCGAAC17900.010.7653641
CGCAAAT33250.010.7428571
CGGAAAT42950.010.7240991
AAATCGA32300.010.6222914
Produced by FastQC (version 0.11.5)