FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22805827
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143250.041.7340394
CAAGTCG106000.024.47547194
TAAGTCG32950.020.82549394
GAAGTCG127800.018.68231894
CGAATCG16050.014.0560753
AATCGTA18450.012.7371285
AAAGTCG58250.012.1836994
GGAAAAT172350.012.162461
CGCATAT9200.011.751
CGCGAGT27650.011.7287521
CGAAAAT41400.011.6932381
GTCGGTT48350.011.5677351
CGGGATT41500.011.5518071
CGAAGAT85100.011.4324331
TATACGC8651.8189894E-1211.4104047
GGCGAAT40800.011.2892161
TCGAATC18900.011.1904762
CGCGTAT5052.4742367E-611.1683161
CTCGTAT17400.011.0747131
CGGAAAT39250.011.0165611
Produced by FastQC (version 0.11.5)