FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23012988
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95900.052.09697394
CAAGTCG82000.026.88170694
TAAGTCG26100.022.50957794
GAAGTCG112250.016.7483394
GTTCGAT29850.015.1155771
TTCGATT31400.013.4713362
GTCGGAT28250.012.1451321
TATACGG17450.011.98567334-35
GGCGAAT52700.011.861481
GTCGTTT20200.011.6336631
CGCGATT8551.8189894E-1211.5438591
GGCGATT36100.011.1966751
GGCGGAT89650.011.16675951
TCGATTC34750.011.0906483
GTCGGGT43000.010.9302321
AAAGTCG43950.010.80090994
GTCGGAG36750.010.6149651
CGCGTAT4952.4164641E-510.4444441
GTCGGTA18200.010.329671
GTATACG18500.010.16216234-35
Produced by FastQC (version 0.11.5)