FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23012988
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122000.040.87458894
CAAGTCG96500.025.42383494
TAAGTCG25900.017.42084994
GAAGTCG120850.014.58419594
CGTATCG18000.012.011113
GTCGGTA17500.011.281
CGCGTAT4601.0732172E-511.239131
CGCGGAT26650.010.7579741
GTCGGGT48450.010.6707941
AAAGTCG50700.010.56804894
GCGGTAT20250.010.4444441
GTTCGAT28200.010.1666671
AATCGTA18050.010.1551245
GGGAAAT159450.010.0809031
CTCGGTT39650.010.0756621
GGCGATT39050.09.9897571
GTCCGAT15750.09.8476191
GCCGGTT51350.09.70204451
GGCGGAT89900.09.5672961
GTCGTTT25600.09.5468751
Produced by FastQC (version 0.11.5)