FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22272125
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG93300.045.7406294
CAAGTCG71750.025.02299794
TAAGTCG29850.021.09882794
GAAGTCG111800.018.16100194
GTCGGTT43600.013.7981661
TACGCTA8300.012.4578319
CGAAAAT32950.012.2670721
GTTTAAT85750.012.2227411
GGAAAAT175950.011.88691
CGAAGAT85600.011.6950931
TTAGCGA18200.011.6208797
TATACGC8105.456968E-1211.60493857
AAAGTCG49550.011.38244294
GTTTTTT271350.011.0853151
GTTTATT96550.011.0502331
CGCAAAT30200.011.0496691
GTCGGAT32000.010.868751
CGGAAAT41200.010.8373791
CTTTAAT102350.010.8373231
GCGGTAT27800.010.8201441
Produced by FastQC (version 0.11.5)