FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22124472
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG129350.060.13529694
CAAGTCG97500.030.99590194
TAAGTCG35350.024.99575894
GAAGTCG138450.023.66125194
AAAGTCG53700.019.08007694
GTTTTTT312800.016.5431581
GCGAAAT31500.014.323811
TTTTTTC400700.013.8994262
GCCGAAT49750.013.7929661
AAAAATC226250.012.9626532
GAAAAAT231350.012.7784741
AAATCCA205900.012.4633324
GTCGAAT15900.012.4150941
TTTTTTG437700.012.1660962
GCGGAAT43300.012.1570441
CTCGATT30550.012.1538471
GTTTAAT97900.011.8580191
CGAAAAC29500.011.7898311
GCCGATT24850.011.7263581
AATCCAC121150.011.599675
Produced by FastQC (version 0.11.5)