FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22371308
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119650.054.48307494
CAAGTCG92350.026.46453594
GAAGTCG129350.021.11093994
TAAGTCG35000.020.81428594
AAAGTCG48500.016.66804194
GTTTTTT281950.015.9694981
GCCGAAT55600.015.6384891
TTTTTTC408150.014.9815032
GCGAAAT27200.014.8602941
TTTTTTG432250.013.7330252
GCGGAAT39750.013.2427671
GTTTAAT90750.013.0512391
GAAAAAT225950.013.0422661
CGCAATT20050.012.8927681
AAATCCA198500.012.8332494
GCGAATT17600.012.5511361
GTTTTAC74850.012.4956581
CGAAAAC33200.012.4578311
AAATCGA32500.012.2923074
CGAATCC44800.012.2745543
Produced by FastQC (version 0.11.5)