FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22371308
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146050.044.69907894
CAAGTCG111900.024.57104794
TAAGTCG33450.022.9028494
GAAGTCG126400.018.88924294
GCCGAAT49850.015.179541
GTTTTTT284900.014.5668651
GCGGTAT27550.014.5009081
CGAAAAC37600.014.1250011
AAAGTCG58350.013.53213394
CGCAAAT27350.013.06032851
CTCGATT32650.012.8116381
CGCGAAC19850.012.7858951
CGCAAAC43400.012.7788021
CGAAAAT37450.011.9225641
GTTTTAT157650.011.5972091
GCGAAAT26750.011.5962611
AAATCGA30600.011.5196084
AAATCCA193500.011.24599554
GGAAAAT179500.011.2328691
GTTTAAT87700.011.2006851
Produced by FastQC (version 0.11.5)