FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22690798
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG70450.037.22640294
CAAGTCG56150.022.51647494
TAAGTCG22100.017.86425294
GTTCGAT32200.015.3260861
GAAGTCG92850.013.00915494
TTCGATT35100.012.9886032
TTAGCGA28100.012.8790038
TATACGG18950.012.52506534-35
TAGCGAG32800.012.3231719
CGCGTAT4254.4457975E-612.1647051
GTCGGAA42450.011.736161
GTCCGAT15700.011.67515851
CGTAACG4205.2624782E-511.1904753
GTCGAAA31300.010.961661
GCGGTAT29650.010.7790891
TCGGATT26200.010.7633592
GTCGTGT38250.010.6901961
GTATACG18750.010.65333334-35
GTTTATT92500.010.5178371
CGTTCGA19000.010.3894737
Produced by FastQC (version 0.11.5)