FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22690798
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101800.025.94695594
TAAGTCG19950.018.1403594
CAAGTCG78450.017.19439194
CGCGTAA8900.015.8426961
CGCGGAT26850.012.7783981
CGCGTAT4851.11584086E-712.5979381
CGTATCG20300.012.2709363
GTTCGAT31850.012.2480371
GTCCGAT14400.012.0763881
GTCGTTT21850.011.8306621
TTCGATT33900.011.6460162
CGCATAT8950.011.5530711
GAAGTCG95200.011.40441194
TCGGATT25350.011.1242592
TAGCGAG29950.010.8280469
GCGGTAT26050.010.82533551
TTAGCGA26800.010.6977618
TATACGG17950.010.47353734-35
CGGGAAT63100.010.2789221
CGCGTGT27500.010.0836351
Produced by FastQC (version 0.11.5)