FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22012407
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG142950.053.55928494
CAAGTCG114650.029.96685894
TAAGTCG36350.024.30811394
GAAGTCG157450.022.29850894
TACGCTA9350.021.1122999
TATACGC9600.020.56257
ATATACG13500.017.4074086
AAAGTCG47800.017.40376794
GGAAAAT160500.012.5626161
CGCGTAT4557.0384704E-712.3956041
GTTTATT90150.012.3560731
CGAAAAT31250.012.33281
CTTTAAT95350.012.2737281
CGCGGAT21900.012.2328771
TAAATCG11950.012.1924693
AAATCGA29050.012.1342524
GTTCGAA45000.011.8022211
GTTTTTT250950.011.7242481
AATCGTA19350.011.65891555
CTTTATT158600.011.5870121
Produced by FastQC (version 0.11.5)