FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22012407
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG168250.044.1087794
CAAGTCG122750.027.33849394
TAAGTCG39900.027.32832194
GAAGTCG142100.021.36664294
TATACGC8450.017.7988177
AAAGTCG59700.015.5879494
TCGGATC49950.014.02002194
TACGCTA11100.013.9729739
GTCGGTT38300.013.3759781
CGAAAAT38650.013.254851
GTCGGAT47050.012.886291594
ATATACG14250.012.8631576
GGAAAAT165000.011.9351511
CGAAGAT87750.011.67635251
CGAATCG15650.011.4121413
GGCGAAT39700.011.3652391
AATCGTA18000.011.22777755
GTTTTAT122900.011.2050451
CTTTATT142550.010.9793051
GGCGATT32250.010.9302331
Produced by FastQC (version 0.11.5)