FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23099994
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132350.042.43671894
CAAGTCG99800.024.15931994
TAAGTCG36950.022.25980894
GAAGTCG123950.020.74142694
CTCGATT43000.018.0348831
CGAAAAC38200.016.11781
GTTTTTT340650.014.8319381
CTTAGAT67650.014.728751
CGCGTTT16700.013.790421
GTTTAAT103100.013.7216291
AAAGTCG57200.013.146852594
CGAAAAT38950.012.9114251
CTCAGAT181050.012.901961
CGAAGAT83250.012.7591591
CGCAAAT33000.012.6757571
CTCGAAT40150.012.5255281
AAATCCA216100.012.331794
TCGAATC19300.012.1761652
GTTTTTC192100.012.1598121
CGGAAAT41450.012.13268851
Produced by FastQC (version 0.11.5)