FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22378867
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128100.053.8977494
CAAGTCG98850.029.66919794
TAAGTCG35500.024.75774894
GAAGTCG137700.020.7182394
AAAGTCG50900.016.25147494
GTCGGTT49250.013.2649751
AATCGTA16000.012.9255
CGAATCG15700.012.8726123
CGGAAAT47250.012.6328051
CGAAAAT31950.012.5039131
GTTTATT96550.012.1698621
CGCATAT7751.8189894E-1212.1290321
CGCGGAT26150.011.68261
GGCGAAT56000.011.6660721
GTTTTTT262800.011.6248111
CTTTATT151350.011.4899241
GTTTTAT145650.011.3587371
TCGGATT29750.011.2168072
CGAAGAT88700.011.1803841
GGAAAAT164900.011.0303231
Produced by FastQC (version 0.11.5)