FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22378867
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151450.044.19148394
CAAGTCG114450.026.52861694
TAAGTCG36750.023.91564694
GAAGTCG133800.019.39013594
CGGAAAT44950.012.5472761
AAAGTCG59500.012.24369894
CGAAAAT44300.012.2009041
TTAGCGA18950.012.1530357
CGAATCG16400.012.0365853
CGAAGAT84050.011.7989291
CGCGAAC14300.011.5034961
CTCAGAT166850.011.2676071
GTTTATT96500.011.2020731
CGCAAAT36300.011.1349871
CTTTATT145550.011.075921
CTCGGTT40500.011.0246911
GCCGAAT47150.010.8653241
CGCAATT22400.010.7008941
AAATCCA188850.010.651844
CGCGGAT28000.010.5750011
Produced by FastQC (version 0.11.5)