FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22325559
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123250.055.98052294
CAAGTCG95250.029.31023494
TAAGTCG39400.024.21573494
GAAGTCG135350.022.22386494
AAAGTCG50550.016.5499594
TACGCTA6800.015.89705859
TATACGC7500.014.4133327
CGAAAAT32850.014.1643841
GTTTTAT147200.012.8036681
GTTTATT104000.012.4278841
GTTTTTT272600.012.3965511
ATATACG11000.012.3909086
GTTTAAT91950.012.3186511
GTCGGTT43250.012.1710971
CGCAAAT29750.012.1647051
CGGAAAT46700.012.0770871
GTCAATT60250.011.8572621
CGAATCG16650.011.8558563
GGAAAAT181450.011.733811
GGCGAAT53300.011.6397741
Produced by FastQC (version 0.11.5)