FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22325559
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG147300.046.7766494
CAAGTCG108650.026.99309594
TAAGTCG38950.023.53016794
GAAGTCG134600.020.70653794
AAAGTCG59650.016.2313594
TATACGC8750.015.047
CGAATCG17950.014.924793
GGCGAAT43450.013.8457991
AATCGTA20950.013.2362765
CGAAAAT40650.013.065191
CGCGATT7057.2759576E-1111.9999991
ATATACG12250.011.8938776
GTCGGTT38750.011.8864511
GTTTAAT74150.011.6628461
CGAAGAT79900.011.4705871
CGGAAAT40450.011.2707041
GGAAAAT167200.011.1596881
GTTTATT107850.011.0690771
CTTTAAT105700.010.8940391
AAATCGA30300.010.8580864
Produced by FastQC (version 0.11.5)