FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21640741
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104500.053.9712994
CAAGTCG85800.029.47086194
TAAGTCG29350.022.89948894
GAAGTCG118300.019.26880894
GTTCGAT28650.015.4205931
GTCGGAT28550.013.4991251
TATACGG15850.012.89905434-35
CGTATCG14800.012.7027033
GCGGTAT23100.012.6147181
TTCGATT32950.012.5523532
CGCGTAA7601.8189894E-1212.3684211
AAAGTCG46300.012.28293794
GTCGTTT22600.012.2699111
TCGGATT25300.011.8893292
AATCGTA24150.011.8716365
ATCGTAT18650.011.5924936
CGCGTAT4508.406625E-611.488891
GTCCGAT14400.011.0972221
GTCGGAA35350.010.5035351
CGGTATA15500.010.3096772
Produced by FastQC (version 0.11.5)