FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21640741
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127500.044.1615794
CAAGTCG102050.025.6531194
TAAGTCG30400.024.73684194
GAAGTCG117950.016.815694
CGCGTAA8350.015.1976051
AAAGTCG58050.012.87338594
CGCGGAT24350.011.388091
GTTCGAT26750.011.06915951
GCGGTAT22100.011.0588231
AATCGTA22050.010.87074855
TTAGCGA20300.010.6502468
GTCCGAT12950.010.5250971
CGGGGTT89700.010.1125971
CGGGAAT53000.09.93207551
CGTATCG18600.09.8548383
CGCGGTT41850.09.770611
ATCGTAT19850.09.70780856
GGCGATT38000.09.5236841
TCGGGTC50550.09.3907022
TAGCGAG27150.09.3480669
Produced by FastQC (version 0.11.5)