FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22868596
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96750.050.57053894
CAAGTCG79300.023.94451394
TAAGTCG28600.022.18531294
GAAGTCG113150.019.43968294
GTCCGAT15400.011.5974031
CGTAACG5809.258292E-811.3448273
GTCGTAT11100.011.0090081
GTCGTTT21450.010.7365961
CGCGTTT14150.010.6289751
AAAGTCG42900.010.40792694
CGCAATT22350.010.304251
TCGGATT26500.010.2867922
CTCGGTT40800.010.2524511
CGCAAAT27900.09.9390681
CGGGAAT50650.09.7433371
GTTTATT109150.09.7315621
CTCGTAT16500.09.4000011
AATCGTA22800.09.2763165
GTCGATT22000.09.1863631
GTCCGTT26800.09.1194031
Produced by FastQC (version 0.11.5)