FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22868596
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122500.040.0171494
CAAGTCG92900.022.61463794
TAAGTCG31900.022.54231894
GAAGTCG117100.016.13492894
CGCGTAT4302.3068424E-814.2093021
CGTATCG16250.012.7261533
TCGGATT27600.011.7499992
GTCGTTT23350.011.0706631
GTCCGAT14100.011.01
AAAGTCG50100.010.88223594
CTCGGAT60100.010.6356071
GTTCGAT27100.09.8856081
GTTTATT117150.09.8693981
CTCGGTT36350.09.8266841
CGGGAAT50850.09.7050141
CGCGTAA7855.8487785E-89.57961751
CCCGTAT28350.09.4497351
CTCAGAT179000.09.4262561
GTATCGA22950.09.4204794
CGCAACT34100.09.3724331
Produced by FastQC (version 0.11.5)