FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22022881
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG93200.034.94742694
TAAGTCG27850.020.5888794
CAAGTCG69550.018.38102194
GAAGTCG92000.016.1434894
CGAAAAT52250.015.5617221
GTTTTTT363900.014.7754881
CGCAATC31350.014.6921861
CGGATAT24650.014.3002041
CTCGATT54350.014.268631
CGAATCG14550.013.8900353
CGCAAAT29400.013.74831
AATCGTA21100.013.5876795
TATACGC8350.013.5089837
CGAGAAC56450.013.1550051
GTTTAAT110350.012.8201191
AAATCCA228100.012.6720754
CGAAAAC32650.012.2358361
CGGAAAT47800.012.1924691
GCGAAAT32150.011.9875581
CAACGCA34950.011.9685287
Produced by FastQC (version 0.11.5)