FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21770730
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107700.056.7316694
CAAGTCG80600.029.73945294
TAAGTCG33650.025.00148694
GAAGTCG127050.024.11963894
GTTTTTT289250.016.2976651
AAAGTCG49650.015.24068594
TACGCTA9200.014.8152179
TTTTTTC417400.014.4805952
GCGAAAT25650.013.1929831
CTCGATT47750.013.09111
GTTTTAC73100.012.7948021
GTTTTAT160250.012.6408741
TTTTTTG455050.012.5388422
GAAAAAT220100.012.4920491
TATACGC9950.012.2814067
TTTTTTA417750.012.2745662
AAATCCA191150.012.2448334
CGAATCG17950.012.0445683
AAAAATC208000.012.043752
CGGAAAT38800.011.9922681
Produced by FastQC (version 0.11.5)