FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21361492
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG133550.062.0449394
CAAGTCG102000.032.0245194
TAAGTCG41050.028.39464294
GAAGTCG144850.026.57438994
AAAGTCG56350.017.93256494
GTTTTTT261800.017.1447681
TTTTTTC323550.014.8604552
CGAAAAT46550.014.8421061
GCGAAAT32250.014.4279081
GTTTTAC57200.014.2972031
AAAAATC184950.013.7226282
GAAAAAT178450.013.4059971
TTTTTTG365400.013.0555562
AAATCCA182600.012.9983584
CGAAATA23950.012.9519832
CGGAAAT42850.012.613771
CGGATAT24500.012.4693881
AATCCAC110500.012.2497745
TATACGC9000.012.0111127
GTTTTCT204550.011.9941341
Produced by FastQC (version 0.11.5)