FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21361492
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG174200.047.64753394
CAAGTCG125950.027.5768294
TAAGTCG38900.027.42673794
GAAGTCG164200.022.6985494
AAAGTCG67250.015.51524394
GTTTTTT251100.015.3859021
CGCGAAT20050.014.7680821
CGAAAAC35650.014.3702671
TACGCTA7850.014.3694289
CGCAAAC46650.014.2057891
AAAAATC183150.013.9601432
CTCGATT46450.013.8622191
GTTTTAT126800.013.2697171
CGCGATT7451.8189894E-1212.6174511
GAAAAAT188400.012.0992581
CGAATTG24200.012.0413243
GCGAAAT37500.012.0320011
GGAAAAT150700.011.9761121
CGGATAT21300.011.9154931
AAATCCA177500.011.8890154
Produced by FastQC (version 0.11.5)