FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21883808
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123050.062.98496694
CAAGTCG108800.033.34926694
TAAGTCG36100.028.25207794
GAAGTCG133400.020.89280394
AAAGTCG47850.015.51933194
GTCCGAT14500.013.9379311
GTCGAAT14100.011.6666671
GTCGATT22800.010.9254391
GTCGGAT27300.010.8461541
CGGAAAT40150.010.7696141
CGCGATT8752.5465852E-1110.7428571
GGCGAAT48550.010.4552011
GTCGGTT41400.010.3309171
TAGCGAG25500.010.32156859
GGCGATT36150.010.2710921
TTAGCGA17500.010.2057148
GTTCGAT26300.010.1863121
GCCGGTT52800.010.1477271
GTCGGGT48650.010.1438851
CGCGTAT4257.2742754E-49.9529411
Produced by FastQC (version 0.11.5)