FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21883808
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG145600.053.29464394
CAAGTCG116150.032.37193394
TAAGTCG35400.025.22598894
GAAGTCG139400.020.36441894
AAAGTCG52200.015.93678194
CGCGTAT4605.588481E-813.2826081
GCGGTAT22500.012.3244451
ATCGTAT14950.012.260876
GGCGATT34900.011.8510031
CTCGGTT39250.011.6152871
AATCGTA18750.011.5306675
CGCGTAA7101.2325472E-810.591551
CGCGAAT9409.094947E-1210.5000011
GTCCGAT13950.010.4444451
GTTCGAT24350.010.22997951
GGCGAAT41400.010.2173911
CTCGTAT14500.010.0482761
GGCGGTT49600.010.0443541
CGCAAAT24150.09.9254671
GTCGGTT40500.09.8641971
Produced by FastQC (version 0.11.5)