FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22944127
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110300.055.05349394
CAAGTCG84800.029.4858594
TAAGTCG32500.024.29538594
GAAGTCG129750.020.61117694
GTTTTTT299100.017.1123371
TACGCTA8250.016.5212129
TTTTTTC381100.016.4271852
AAAGTCG46400.015.19396694
CGAAAAT34800.014.4511491
CGCGAAT14300.014.1328671
TATACGC8100.013.9259267
TTTTTTG393150.013.8674812
CGCGAAC10450.013.4928241
GGAAAAT165500.013.4610261
CTCGATT44100.013.215421
GAAAAAT211050.012.2928221
GTCGATT22950.012.2875811
AAATCCA201150.012.126774
GTTTTTC159400.012.0890841
CGCGTTT16100.011.9689451
Produced by FastQC (version 0.11.5)