FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22944127
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139600.043.464994
CAAGTCG104050.025.47621394
TAAGTCG34850.021.98278494
GAAGTCG127250.019.16935294
GTTTTTT281600.014.52059651
CGCAAAC42900.013.9137531
AAAGTCG56950.013.78226694
CGAAAAT42500.012.60705951
TATACGC8650.012.4971097
CGCGAAT12850.012.4357981
CTCGATT41050.012.0219241
CGAAAAC36050.011.99445151
CGCAATT24050.011.7255731
AAATCCA198150.011.5513514
GGAAAAC149450.011.4787551
GGAAAAT162100.011.4238131
CGAATCG16250.011.283
GTTTTCT209200.011.1434031
CGAAGAT87200.010.9415131
AAAAATC192250.010.9035112
Produced by FastQC (version 0.11.5)