FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22949726
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96450.049.7045194
TAAGTCG29750.026.54117694
CAAGTCG80300.023.9389894
GAAGTCG117800.018.35314294
TTTTTTC426350.016.0506632
CGAAAAT33050.015.074131
TTTTTTG471700.014.9658682
GTTTTTT288150.014.5656771
CGAAAAC33050.014.2208781
CTCGATT43150.014.1599071
TTTTTTA449300.013.9755172
AAAGTCG42150.013.93831594
CGCAAAC39700.013.2594451
GTTTTAC65400.013.2232421
CGCATAT7850.013.1719751
CTTAGAT66900.013.1375191
GGAAAAT169600.013.1356121
CGCAAAT31350.012.8931421
CGAATCG18050.012.4986153
GCGAAAT27650.012.408681
Produced by FastQC (version 0.11.5)