FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22949726
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125850.037.9062494
CAAGTCG96750.022.4434194
CGCGAAC12150.018.5679021
TAAGTCG33200.018.54518194
GAAGTCG113350.017.7053494
CGCGAAT13450.017.4721181
GTTTTTT282800.014.8246121
CTTAGAT64050.014.2357531
CGGATAT22700.014.0792951
CGAAAAC37900.013.269131
CGCAATT21500.013.1162791
GTTTTAT149100.012.9242121
GTTTAAT89850.012.8158041
CTTTAAC74450.012.4996631
AATCGTA22200.012.4909925
CGCATAT7551.8189894E-1212.4503321
CGCAAAC43050.012.3368181
CGAATCG19100.012.3036643
AAATCCA200550.012.2333584
AAAGTCG57500.012.17913194
Produced by FastQC (version 0.11.5)