FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22159750
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115550.051.0064994
CAAGTCG90050.026.82731894
TAAGTCG34300.024.80174694
GAAGTCG131000.019.37404494
CGAATCG16950.015.2507363
AAAGTCG53350.015.15276394
CGAAAAT30450.013.5829231
CGCAAAT30400.012.52302551
CGGAAAT47150.012.4602331
GTTTATT98850.012.4097111
GGCGAAT38650.012.4036221
GTTTTTT265650.012.0662521
TACGCTA5951.03773345E-811.848749
GTCCGAT14650.011.5494881
GTCGTAT11600.011.3448281
AATCGTA24650.011.2494935
TATACGC8106.91216E-1111.0246927
GTTTAAT83250.011.0090081
GTCGGTT42350.010.9870121
GGAAAAT191600.010.94051
Produced by FastQC (version 0.11.5)