FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21890650
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99950.048.76338294
CAAGTCG80700.024.8104194
TAAGTCG34250.022.36788494
GAAGTCG111350.021.27346294
CGAATCG17750.016.4169033
GTTTTTT312150.015.10203551
TTTTTTC451700.013.58910752
GCGAAAT27000.013.5777791
AAAGTCG47600.013.32983394
TATACGC10450.013.0430637
AAATCGA33900.012.4778784
GGAAAAT194350.012.4059691
CTCGATT43200.012.402781
TACGCTA10500.012.0857139
GAAAAAT234500.011.9654591
CGCAAAT29100.011.951891
TTTTTTG493150.011.9418032
CGAAGAT73800.011.9092151
GTTTTAT169000.011.8751491
TTTTTTA461150.011.8430012
Produced by FastQC (version 0.11.5)