FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21890650
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126550.039.0707394
CAAGTCG92500.023.1697394
GAAGTCG112500.020.01155594
TAAGTCG36950.019.8430394
TATACGC8900.015.3146087
CTCGATT41250.014.3563641
CGAAAAC38600.013.880831
CGAATCG16500.013.6727283
GTTTTTT293100.013.5660191
AAATCGA33350.012.9655184
AAATCCA208200.012.3256494
TACGCTA10300.012.3203899
GTTTAAT99250.012.3123441
CGCAAAT30850.012.1880071
TTTAATC116300.012.0834062
CTCAGAT174200.012.0063151
GGAAAAT193000.011.9813481
CTCGAAT37800.011.812171
GTTTTAT158650.011.7018591
CGAAAAT39500.011.660761
Produced by FastQC (version 0.11.5)