FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23783007
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG133100.056.0751394
CAAGTCG99350.031.03371894
TAAGTCG38400.026.31510494
GAAGTCG143700.022.50243894
AAAGTCG55500.017.1909994
CGAAAAT41450.015.3075991
TTAGCGA26850.015.2290517
GTTTTTT318350.014.8669711
TAGCGAG31500.014.0253968
TTTTTTC386900.013.909282
GTTTAAT99800.012.8096191
CGCAAAT30450.011.8850571
TTTTTTG434700.011.7418912
GAAAAAT231900.011.73481
GTTTTCT227600.011.6880491
GCGAAAT32450.011.4422191
GTCGAAT16850.011.4362021
AATCCAC115550.011.3483345
CTCGATT44000.011.3227271
GCCGAAT55900.011.1824691
Produced by FastQC (version 0.11.5)