FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23231863
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85700.047.00000494
CAAGTCG70900.026.5162294
TAAGTCG25100.021.53386594
GAAGTCG107400.017.32960994
GTTCGAT29050.014.7228921
TTCGATT30750.012.5333342
GTCCGAT14650.012.1911261
GTCGTTT19250.011.4753271
TTAGCGA22250.011.4067428
AATCGTA18500.010.9243255
GGCGAAT59450.010.7518921
AAAGTCG40700.010.73955794
TCGGATT25750.010.5864092
GTTTATT111450.010.5428451
ATCGTAT15200.010.5131596
CGCGAAT8652.3283064E-1010.32371
GTCGAAT12350.010.2753041
CGCGTAA7855.3987605E-910.1783451
GTCGGAA35300.010.1189811
TAGCGAG28600.010.0244759
Produced by FastQC (version 0.11.5)