FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21439102
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG158000.063.12278794
CAAGTCG125850.032.4163794
TAAGTCG40150.030.31880294
GAAGTCG159400.026.92032694
AAAGTCG53950.019.60148294
GCGAAAT25200.017.9047621
TTTTTTC326600.017.7725032
GTTTTTT247650.016.2075521
GAAAAAT177550.014.2680931
CTCGATT34900.014.0057311
GCGGAAT40050.013.9650441
TTTTTTG366300.013.1902812
GCCGAAT50850.012.940021
AAAAATC172000.012.8156982
AAATCCA164100.012.6593544
GCGGTAT26700.012.4981281
GTCGATT20850.012.3980821
GTTTTTC139900.012.3967111
TCGATTG21800.012.0733962
TCGAATC15600.012.0512822
Produced by FastQC (version 0.11.5)