FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21439102
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG186700.053.4193994
CAAGTCG144800.030.99792794
TAAGTCG40650.028.78966794
GAAGTCG160400.024.43765694
AAAGTCG63050.017.59238894
GTTTTTT247900.014.9588541
GCGAAAT24700.014.0809721
GCCGAAT51850.014.0501451
GCGGTAT25250.013.58811951
CGCGAAT7401.8189894E-1212.70270251
TACGCTA7800.012.6538469
AAATCCA169800.012.538874
AAATCGA25600.012.4843744
GTTTAAT68850.012.0145241
GCGGAAT40900.011.95111
TTTAATC85550.011.8118062
AATCCAC98150.011.7320435
AAAAATC162650.011.7030432
GTTTTCT184100.011.6925571
GAAAAAT169300.011.6320151
Produced by FastQC (version 0.11.5)